Micro: Enterobacteriacea

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created 8 years ago by jgaversa5
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1

what is the first differentiation of gram negative bacteria

cocci and bacilli

2

how are gram negative bacilli first divvidied

on air growth (many) or not (bacteroides or fusobacterium)

3

gram negative, air growing bacteria are then separated by what?

oxidase

4

what are the oxidase positive, air growing gram negative bacilli

GNR related to GI and respiratory tract
GNR related to animal sources
non fermenters

5

how are gram negative, air growing bacteria that are oxidase negative next separated

by glucose metabolism

6

what are the glucose fermenting, oxidase negative, air growing gram negative bacilli

enterobacteriaceae

7

what are the glucose inactive (or OTHER metabolizing) oxidase negative, air growing gram negative bacilli

acinetobacter
calcoaceticus

8

what are the characteristics of enterobacteriacae

all facultative anaerobes
all ferment glucose
none have cytochrome oxidase
reduce nitrates to nitrites

9

where are enterobacteriaceae found primarily

colons of animals

10

do enterobacteriaceae cause a wide variety of infections

yes

11

what types of media do enterobacteriaceae get inoculated on

blood agar plate

selective differentiial media (MacConkey's agar or EMB agar)

12

what is a very importnat way to distinguish initial identification of enterobacteriaceae

lactose fermentation

13

what are used to make species identification of enterobacteriacaea

biochemical reactions

14

what is the O antigen of enterobacteriaceae from

lipopolysaccharide O side chains

15

what is the K antigen of enterobacteriaceae

capsule

16

what is the H antigen of enterbacteriaceae

flagella

17

what are the rapidly lactose fermenting enterobacteriacae

escheria
enterobacter
klebsiella

18

what are the slowly lactose fermenting enterobacteriaceae

edwardsiella
serratia
citrobacter
arizona
providencia
erwinia
vibrio

19

what enterobacteriaceae do not ferment lactose

shigella
salmonella
proteus
pseudomonaas

20

aside from lactose fermentation what is another reaction for screening enterobacteriaceae

triple sugar iron agar reaction

21

what are stools like in secretory/watery diarrhea

watery, copious, no blood or WBC

22

is there invasion in secretory or watery disahea

no

23

what things cause secretory diarrhea

v. cholerae
e. coli (ETEC)

24

what are stools like in dystentery

scant volume, lots of blood and WBCs

25

is there invasion in dystentary

yes

26

what things cause dysentery

shigella
entamoeba histolytica

27

what is bloody watery diarrhea like

moderate volume with some blood and WBCs

28

is there invasion in bloody watery diarrhea

yes

29

what things cause bloody watery diarrhea

salmonella
campylobacter
yersinia

30

what is the stool like in hemorrhagic colitis

bloddy, no WBCs

31

is there invasion in hemorrhagic colitis

no

32

what causes hemorrhagic enterocolitis

E coli O157

33

how are enterobacteriaceae grouped sometimes

biochemical and epidemiological similarities

34

what is salmonella grouped with in terms of epidemiological similarity

salmonella
arizona
citrobacter
edwardsiella

35

what is e. coli grouped with epidemiologically

nothing

36

what is klebsiella grouped with epidemiologically

enterobacter and serratia

37

what is proteus grouped with epidemiologically

morganella
providencia

38

what are the GNRs associated with GI disease

e. coli
salmonella
edwardsiella
arizona
shigella
vibrio
campylobacter
h. pylori
aeromonas
plesiomonas

39

what are the GNRs associated with urinary system infections

E. coli
citrobacter
proteus
morganella
providencia
klebsiella
enterobacter
serratia

40

what are teh GNRs associated with respiratory tract infections

h. influenza
legionalla
bordetella pertussis
moraxella
klebsiella
enterobacter
serratia

41

what are the GNRs associated with animal sources

brucella
fracnsicella tularensis
yersinia pestis
pasturella multocida

42

what is a GNR and a non-fermeter

pseudomonas