1/19 and 1/24 Immunology Flashcards


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1

steps for ELISA

  1. protein of interest is bound to wells
  2. blocking - coat the rest of the plate
  3. Abs added, primary, secondary
  4. Substrate added, color change
  5. Data collected

2

Purpose of Immunofluorescence

finding location of specific protein in cell

3

Purpose of Flow Cytometry

categorizing cells based on size, granularity, protein expression

4

gating

selection of subpopulations for analysis

5

disadvantages of flow cytometry

no standardized methods, subjective nature

6

PRRs

pathogen recognition receptors, (think in relation to PAMPs)

7

types of dendritic cells

cDC and pDC

8

cDC

conventional dendritic cell, resident

9

pDC

plasmacytoid dendritic cell, good at antiviral

10

signaling receptors

bind to pathogen and phagocytose, or lead to phagolysosome

11

C-type lectins

mannose receptor and Dectin-1, recognize carbohydrates

12

scavenger receptors

look for damaged low density lipoproteins

13

CR

complement receptors

14

what dendritic cells present antigens to

T cells in the lymph node

15

general steps in receptor mediated endocytosis

receive, bind, engulf, break down

16

bactericidal enzymes

lysozyme, acid hydrolases

17

what ligands tend to be

carbohydrates, lipids, proteins, nucleic acids

18

steps for phagocytic and signaling receptors

recognize, intracellular signaling, gene expression (cytokines)

19

what Toll and TLRs are associated with

embryonic development, resistance to many types of infections

20

common location of TLR-3, TLR-7, TLR-8, TLR-9

endosome so they can kill bacterial and viral nucleic acids

21

where a TLR that recognizes nucleic acids must be

inside of the cell

22

what TLR-2 recognizes

lipopeptide

23

LLR domain

horseshoe shape in TLRs, recognition domain

24

TIR domain

toll interleukin receptor domain, signaling part of TLR

25

what TLRs have to do to be functional

dimerize

26

what kind of dimers most TLRs make

homodimers

27

what dimer TLR-2 makes

heterodimers

28

what TLRs are MyD88-dependent

all TLRs except TLR-3 and sometimes TLR-4

29

significance of ubiquitin in TLR signaling

usually it tags things for degradation, but in this process it tags things for activation

30

what MyD88-dependent TLR signaling uses as a scaffold

recruited MyD88

31

what MyD88-independent TLR signaling uses as a scaffold

recruited TRIF

32

steps in MyD88-dependent TLR signaling

recognition by TLR dimer, MyD88 is recruited, IRAKs are recruited, TRAF-6 is recruited and activated, kinases are activated, IkB is phosphorylated and degraded, TF is phosphorylated, adaptive response

33

steps in MyD88-independent TLR signaling

recognition by TLR dimer, TRIF is recruited, TF --> make interferons, antiviral response

34

TF phosphorylated in MyD88-independent signaling

IRF-3

35

TF phosphorylated in MyD88-dependent signaling

NFkB

36

CARD

domain in NOD-like receptors that generally relates to caspases, but in this case acts as a signaling domain in the recognition of viral nucleic acids

37

RLRs

recognize viral nucleic acids, result in production of type 1 interferons

38

LRR

domain in NOD-like receptors, for recognition

39

NOD

domain in NOD-like receptors, for oligomerization (making dimers)