Microbiology Chapters 6

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Microbiology
Chapter 6
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1

Microbial growth

and increase number of cells

2

Bacteria generally divide by what?

binary fission or budding

3

Phases of growth:
1.lag
2.log
3.stationary
4.decline

1. cells actively produce ATP preparing for cell division
2. greatest cell division (generation time: population doubles)
3. new cells produced as others die
4. fewer cells divide and more cells die

4

involution

exhibits unusual shapes

5

endospores

made for survival

6

colony

formed when a single cell divides to produce a large number of cells. Grow fast in the center and slower around the edges

7

Ways to count bacterial growt

serial dilution and standard plate counts
direct microscopic count
most probable number
filtration
turbidity

8

acidophiles

acidic pH leves

9

neutrophiles

neutral pH leves

10

alkaliphiles

basic pH leves

11

psycholphiles

cold temperatures

12

mesophiles

medium temperatures

13

thermophiles

hot temperatures

14

aerobes

loves free oxygen concentration

15

anaerobes

hates free oxygen concentration

16

microaerophile

likes limited free oxygen concentration

17

obiligate

has to have its required needs; nothing more, nothing less

18

faculative

likes requested conditions but can adapt if need be

19

Most vegetative cells can live only a few hours without what?

moisture

20

hydrostatic pressure

pressure exerted by standing water

21

halophiles

salt loving bacteria

22

osmotic pressure

environments that contain dissolved substances exert this

23

radiation

causes mutations by gamma rays, ultra-violent light, etc

24

CPONCH

adaptation to limited nutrients

25

quorum sensing

explains some peculiar aspects of bacterial luminescence

26

biofilm

layer of one or more kinds of bacterial growth on a surface

27

sporation

formation of endospores

28

culturing bacteria

streak and pour plates

29

culture media:
natual
synthetic

soul, water, living tissue
man-made

30

defined

specific amounts of all substances are known

31

complex

know substances but amounts vary

32

selective

encourages growth of some but not all

33

differential

shows differences by color or pH

34

enrichment

special nutrients to allow growth

35

barophile

lives at high temperatures but die at room temp

36

stock cultures

makes every other culture but never used in lab

37

preserved culture

in dormant state

38

reference culture

preserved culture the way it was first foudn