Smear preperation and simple staining lab 7 Flashcards


Set Details Share
created 10 years ago by vriddle
1,256 views
show moreless
Page to share:
Embed this setcancel
COPY
code changes based on your size selection
Size:
X
Show:
1

Bacterial smear

Dried preperation of bacterial cells on glass slide

2

Properly prepeared bacterial smear

Bacteria are evenly spread out on slide so that they are separated from one another
Bacteria not washed off during staining
Bacterial form not distorted

3

What are the two purposes of heat fixing

Bacteria are fixed to slide
Kills bacteria

4

Purpose of simple staining

Simple easy to use
Gives info about cell shape, size and arrangement

5

Why are positively charged dyes more successful in staining bacteria than neg charged dyes

Bind to bacterial cells by ionic reactions and have positively charged groups that bind to negatively charged molecules like nucleic acids, proteins and on surface of prokaryotic cells

6

Why is time an imp factor in simple staining

Bc you need to make sure you've left on long enough for chemical reaction to occur

7

Why should you use an inoculating needle whe making smears from solid media?a loop when liquid media?

Needle...to break up bacteria can't use needle with liquid bc don't get enough
Loop....make sure you don't get too many bacteria creating difficulty seeing under mic