Bacterial smear
Dried preperation of bacterial cells on glass slide
Properly prepeared bacterial smear
Bacteria are evenly spread out on slide so that they are separated from one another
Bacteria not washed off during staining
Bacterial form not distorted
What are the two purposes of heat fixing
Bacteria are fixed to slide
Kills bacteria
Purpose of simple staining
Simple easy to use
Gives info about cell shape, size and arrangement
Why are positively charged dyes more successful in staining bacteria than neg charged dyes
Bind to bacterial cells by ionic reactions and have positively charged groups that bind to negatively charged molecules like nucleic acids, proteins and on surface of prokaryotic cells
Why is time an imp factor in simple staining
Bc you need to make sure you've left on long enough for chemical reaction to occur
Why should you use an inoculating needle whe making smears from solid media?a loop when liquid media?
Needle...to break up bacteria can't use needle with liquid bc don't get enough
Loop....make sure you don't get too many bacteria creating difficulty seeing under mic