Bailey & Scott's Diagnostic Microbiology: Clinically Significant Yeasts and Yeast-Like Organisms Flashcards


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1

Saccharomyces cerevisiae

the "working yeast"-bread, beer, wine; occasionally normal flora; increasingly isolated from immunocompromised patients-rare pulmonary infection and endocarditis

2

Saccharomyces

creamy, smooth, moist

3

Saccharomyces

yeast cells and short psuedohyphae; asci and ascospores

4

Cryptococcus neoformans

associated with meningitis and pulmonary disease; surrounded by polusaccharide capsule that gives mucoid appearance, protects yeast from environmental stress, not recognizable by phagocytes

5

Cryptococcus gatti

found in variant forms associated with meningitis and pulmonary disease; surrounded by polusaccharide capsule that gives mucoid appearance, protects yeast from environmental stress, not recognizable by phagocytes

6

India ink stain

stain used to detect Cryptococcus capsule by delineating the large capsule

7

blastoconidia only-no hyphae

Cryptococcus result on cornmeal agar

8

urease positive

Cryptococcus biochemical test

9

phenol oxidase

Cryptococcus results is melanin production on caffeic acid/birdseed producing dark color; 4 hour caffeic acid disk test

10

Cryptococcus

smooth white to tan, mucoid, and creamy

11

routine fungal media without cycloheximide

cultivation of Cryptococcus species

12

rapid urease

the basis for presumptive identification of Cryptococcus

13

Rhodotorula

pink to coral or bright orange to red colored colonies, sometimes mucoid

14

Rhodotorula

round to oval blastoconidia with possible rudimentary pseudohyphae

15

capsule production, urease positive, and some nitrate positive

factors that make Rhodotorula similar to Cryptococcus

16

Rhodotorula

not a common agent of disease, but do cause some opportunistic infections associated with fungemia, endocarditis, and keratitis

17

Trichosporon

has 37 valid species that can cause disseminated disease, skin lesions, endocarditis, endophthalmitis, brain abscess, and respiratory infections; can cause white piedra in immunocompetent patients

18

Amphotericin B

antibiotic Trichosporon is resistant to

19

Trichosporon

numerous arthroconidia with round ends and few blastoconidia on cornmeal; septate hyaline hyphae and pseudohyphae

20

Trichosporon

cream-colored, heaped, and dry-to-moist wrinkled colonies

21

urease positive

useful test of biochemical test of Trichosporon along with substrate utilization profile

22

Geotrichum

normal microbiota that causes infections in immunocompromised patients; isolated from lungs, blood, oral cavity, intestinal tract, vaginal tract, and skin; may cause thrush; associated with wounds

23

Geotrichum

arthroconidia and absent blastoconidia

24

Geotrichum

white, moist, with peripheral hyphae and short, white cotton aerial mycelium

25

urease negative

useful biochemical test on top of carbohydrate profile for Geotrichum

26

Malassezia

normal skin flora in 90% of humans; catheter related infections in neonates and patients on long term IV lipids; causes tinea versicolor- brownish scaly lesions on light-skinned persons, disseminated infection in young children

27

Malassezia pachydermatis

recovered from skni lesions; may cause fugnemia in patients who are immunocompromised

28

Malassezia

cream/brown wrinkled

29

Malassezia

yeast-like cells, oval-shaped with monpolar budding, spaghetti and meatball hyphal arrangement; lipophilic

30

C. krusei, parapsilosis, kefyr, and tropicalis

four yeasts identified by pseudohyphae and blastoconidia only

31

C. glabrata and C. neoformans

two yeasts identified by blastoconidia only

32

T. beigelii and G. candidum

two yeasts identified by arthroconidia