Bailey & Scott's Diagnostic Microbiology: General Identification of Yeasts Flashcards


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1

summary of common pathogens

58.2 page 796

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yeasts

eukaryotic, unicellular, round to oval fungi; reproduce asexually and sexually

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blastoconidia

asexual budding of yeast

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ascospores or basidiospores

sexual reproduction of yeast

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moist creamy, pigmented, melanized or hyalin

macroscopic colony morphology of yeast

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conidia

microscopic characteristic in identification of yeast

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positive germ tube

outgrowth produced by spores of spore-releasing fungi during germination that will eventually develop into hyphae

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pseudohyphae

constriction that results from incomplete separation of budding yeast-sausage links

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cornmeal agar

demonstrates production of reproductive structures; induces formation of budding yeast, arthroconidia, pseudohyphae, true hyphae, and chlamydoconidia

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blastoconidia

budding yeast

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arthroconidia

block like yeast

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pseudohyphae

irregular with constrictions

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true hyphae

regular, without constrictions

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chlamydoconidia

round thick walled

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carbohydrate utilization

allows for differentiation of assimilation vs fermentation

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phenoloxidase

detected using niger seed agar and produces a black pigment

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urea agar

assists in biochemical identification; negative test stays pale orange, positive is bright pink with growth; Cryptococcus, Candidas krusei and lipolytica positive

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cornmeal agar

stimulates conidia formations in many fungi; source of nitrogen, carbon, and vitamins, dextrose can be added

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Candida albicans

produces terminal chlamydospores (pathognomic) in cornmeal agar

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carbohydrate assimilation

use of carbon or nitrogen source in presence of oxygen; growth in medium, pH sift and/or color change depending on type of medium; does not always result in fermentation; use of McFarland standard, broth added to tube, incubated at 37 for 7-14 days; negative shows no growth

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carbohydrate fermentation

production of gas showing use of carbon or nitrogen source in the absence of oxygen; also assimilated; inoculated and grown in purple broth base for 24 hours at 37, then subculture and reincubate; overlay with mineral oil, incubate up to 10 days at 37; positive reactions in purple appear yellow (acidic) with the presence of gas