Bailey & Scott's Diagnostic Microbiology: Laboratory Techniques in Mycology: Indirect Examination and Cultivation Flashcards


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1

direct inoculation

adding several drops of liquid specimen to media or streaking of solid media

2

chloramphenicol, gentamicin, and cyclohexamide

three inhibitory substances used for fungal isolation

3

brain heart infusion

media made up of animal tissue enriched with dextrose and sodium chloride to enhance recovery of fastidious pathogenic fungi (Histoplasma and Blastomyces); antibiotics added to inhibit bacteria and rapidly growing molds; blood can further enrich

4

brain heart infusion biphasic blood cultures (Castaneda's method)

media used to recover fungi from blood; broth/agar, peptone, glucose and disodium phosphate; contains liquid broth and solid agar

5

chromogenic agar

selective and differential media for presumptive identification of genus Candida from primary plates; colony morphology and color vary by species; peptone, chloramphenicol, and mix of other chromogenic substrates

6

Candida albicans

shows light to medium green on chromogenic agar

7

Candida tropicalis

shows light blue to metallic blue on chromogenic agar

8

Candida krusei

shows light rose with whitish border on chromogenic agar

9

inhibitory mold agar

enriched fungal slant media containing chloramphenicol to inhibit contaminating bacteria but no cycloheximide; nutrients provided by peptones, derived from casein and animal tissue, and yeast extract, dextrose, starch, and dextrin

10

yeast extract

rich source of vitamins included in media to allow fungi to thrive

11

dextrose, starch, and dextrin

added to fungal media to provide energy sources for metabolism of fungi

12

sodium chloride and metallic salts

added to fungal media to provide essential electrolytes and minerals

13

cycloheximide

used in fungal media to inhibit most saprophytic molds

14

chloramphenicol

broad spectrum antibiotic added to fungal media that inhibits wide range of gram positive and negative bacteria

15

potato (flake or dextrose) agar

supports growth of most clinically encountered fungi; may stimulate growth of conidia and pigment; two peptones, casein and animal tissue and yeast extract, dextrose, starch, and dextrin, sodium chloride and metallic salts, and chloramphenicol

16

mycosel agar

selective medium containing chloramphenicol and cycloheximide used to isolate fungi; nutrients supplied by peptone prepared from soybean meal; utilizes dextrose as energy source for metabolism

17

sabouraud dextrose agar

used for isolation, cultivation, and maintenance of non-pathogenic and pathogenic species of fungi and yeast; pH adjusted to 5.6; utilizes peptone, chloramphenicol and/or tetracycline, gentamicin, and Emmon's modification

18

Emmon's modification

use of only 2% glucose and a neutral pH to grow fungal elements

19

SABHI broth- Sabouraud dextrose and brain heart infusion

medium that utilizes the strengths of two different media that are enriched for isolation of Cryptococcus spp and thermally dimorphic fungi

20

tease preparation

organism removed directly from culture and picked apart with needles

21

scotch tape preparation

tape pressed onto plate and transferred to microscope slide

22

slide culture

organism subbed to small piece of agar, covered with coverslip, then growth removed from coverslip and examined; best indirect microscopic method

23

table 5.1

specimen collection and processing