Lab 1- Microscope Flashcards


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created 10 years ago by acyriac
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A- Rotating nosepiece
B- Ocular lens
C- Substage light

2
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D- Head
E- Objective lens
F- Light control

3
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G- Mechanical stage
H- Stage
I- Condenser

4
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J- Arm
K- Coarse Adjustment Knob
L- Mechanical Stage Control

5
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M- Iris Diapgrahm Lever
N- Fine Adjustment knob
o- Base

6

The proper technique for transporting the microscope

Hold it in an upright position with one hand on the arm and the other arm on the base

7

The microscope must be cleaned with

Special grit free lens paper (In a circular motion)

8

When beginning to focus the ____ power lens should be used.

Lowest power

9

When focusing, always focus ______ from the specimen

Away
(So the slide won't break)

10

use the Coarse Adjustment Knob only with the _____ power lens.

Lower- power lens

11

The platform on which the slide rests for viewing

Stage

12

The microscope structure
used to increase the amount of light passing through the specimen

Condenser

13

The microscope structure
that secure the slide to the stage

Mechanical Stage

14

The microscope structure
that delivers a concentrated beam of light to the specimen

Iris diaphragm

15

The microscope structure
used for precise focusing once initial focusing has been done

Fine Adjustment Knob

16

The microscope structure
that carries the objective lenses; rotates so that different objective lenses can be brought into position over the specimen

movable nose piece

17

The real image is magnified to produce the ______________ seen by your eye.

Virtual Image

18

Define Resolution

The ability to discriminate 2 close objects as separate (Resolving power)

19

The distance from the bottom of the objective lens to the specimen is ____________

Working distance

20

Assume there is an object on the left stage of the field that you want to bring to the center.
What direction would you move the slide?

Right

21

The area of the specimen seen when look through the microscope is _______

field

22

If a microscope has a 10x ocular and the total magnification at a particular time is 950x,
the object lens used at the time is ___x

95
[10x * 95= 950x]

23

Why should the light be dimmed when looking at the living cell?

To provide more contrast for viewing the lightly stained cells.
(Also, a wet mount can dry out by the heat from the light.)

24

If, after focusing in low power, only the Fine Adjustment need be used to focus the specimen at the higher powers, the microscope is said to be _______

Parfocal

25

Say, you are observing an object in the low- power field. When yo switch to high power, it is no longer in your field of view.
Why?

BECAUSE, In lower power you have a WIDER field of view.
When you switch to higher power, your view NARROWS, and becomes out of focus.

Lower power- Wider view
Higher power- Narrow view

26

How can you prevent it from happening?

Center the object as much as possible before switching to a higher power and increasing the magnification slowly.

27

Higher Magnification, Resolution (Increases/ Decreases)

increases

28

Higher magnification, Working distance (Increases/ Decreases)

Decreases

29

Higher magnification, Amount of light needed (Increases/ Decreases)

Increases

30

Higher Magnification, Depth of light (Increases/ Decreases)

Decreases

31

A higher lens is a high power, so it only needs a _______ workiing distance.

shorter (mm)

32

Probable situations arising in microscope:
-> Only half of the fields illuminated because....

-> Field does not change as mechanical stage is moved...

-> because there is a blockage in the light path

-> mechanical stage contacts between the specimen and the objective lens