BIOCHEMICAL TESTS I

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1

MacConkey agar is a selective and differential media used for the isolation and differentiation of ?

non-fastidious Gram-negative rods, particularly members of the family Enterobacteriaceae and the genus Pseudomonas.

2

Mannitol salt agar (MSA) is both a selective and differential medium used in the isolation of?

Staphylococci. It contains 7.5% sodium chloride (NaCl) and thus selects for Staphylococci which cantolerate the osmotic pressure created by high salt concentration

3

The catalase test facilitates the detection of the enzyme catalase in bacteria. It is essential for differentiating?

catalase-positive Micrococcaceae from catalase-negative Streptococcaceae. Some have reported its value in the presumptive differentiation among certain Enterobacteriaceae.

4

The catalase testis also valuable in differentiating?

aerobic (such as Bacillus) and obligate anaerobic bacteria (such as Clostridium), as anaerobes are generally known to lack the enzyme.

5

The catalase enzyme serves to neutralize the bactericidal effects of hydrogen peroxide. Catalase expedites the breakdown of hydrogenperoxide (H2O2) into?

water and oxygen (2H2O2 + Catalase → 2H2O + O2). This reaction is evident by therapid formation of bubbles.

6

The oxidase test is a biochemical reaction that assays for the presence of?

cytochrome c oxidase, an enzyme sometimes called indophenol oxidase.

7

What does SIM deep mean?

Sulfur Indole Motility deeps

8

What does o-f mean?

Oxidation-Fermentation Test