Perfusion - Blood collection and processing for autotransfusion
name the 3 components of blood collection system.
2. Suction tip
Aspiration techniques are similar for unwashed and washed blood recovery
The anticoagulant to blood ratio for heparin is ___mL heparinized saline to ___mL of recovered blood
15 mL of heparnized saline to 100 mL of recovered blood
Aspiration techniques are similar for unwashed and washed blood recovery.
Hemolysis can be caused by small-bore suction tips and coaspiration of air with blood increasing turbulence and shear forces during aspiration.
List the 3 functions of blood reservoir.
1. filter large debris
2. defoamer breaks down air
3. storage - collect blood
Two-port reservoirs are primarily used for autotransfusion
Four port reservoirs can be used for autotransfusion and heart-lung bypass
Most collection reservoirs have gross filtration with a ____micron clot screen
120 micron clot screen
For patient reinfusion of recovered, washed red blood cells, a ____micronaggregate filter is recommended.
Vacuum levels should not exceed -200mm Hg. A vacuum setting of 80 to 150 mm Hg is recommended.
When the red blood cells are washed for patient reinfusion, all contaminants are removed. Therefore, autotransfusionists are exempt from the following universal precautions for handling blood.
List the following blood components in the order of their density.
less dense to more dense
1. Plasma, 2. Leukocytes, 3. Erythrocytes
When blood components separate in a centrifuge bowl, red blood cells comprise the innermost layer
Red blood cells are more dense therefore, they will compose the outermost layer
The fill rate will affect the hematocrit of the washed red blood cell unit because the red blood cells have less time to pack when run at a faster rate than recommended.
The centrifuge in Latham bowls spins at approximately ___rpm and the autoLog centrifuge spins at ___rpm for the separation of blood components.
5600 rpm / 10,000 rpm
For the autoLog bowl, the second fill rate is based upon the condition of the blood entering the bowl and is determined by the software.
It is dependent on the hematocrit
In Latham-design technology, what 2 factors have a profound effect on the quality of the end-product?
wash speed and wash volume
Wash rates should never exceed the fill rate of the bowl.
For the Latham bowl systems. 95% or greater plasma washout is achieved with a volume approximately _____times the bowl.
For orthopedic cases, the wash volume recommended is _____times the volume capacity of the bowl.
3 to 4 times the bowl
for orthopedic its 7 to 10 times
For safe practices, washing cells in a partially filled bowl is recommended.
Name the two types of anticoagulant
1. Heparin - potentiates the activity of antithrombin III
2. Citrate-based solutions