Professor Allen's Tool Chapter Flash cards

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These flash cards go over the tools you use in a microbio lab.
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microbiology
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1

The primary stain used in the endospore stain.

Malachite Green

2

___ is the ability of a microscope to distinguish two points at a specified distance.

resolution

3

_____ dyes have a positively charged colored ion.

Basic

4

The term used for nutrient agar which is allowed to harden at an angle.

slant

5

With the 10X ocular lens in place, what would be the total magnification if the 40X objective lens were used?

40X*10X= 400X

6

Mycobacteria are stained with the acid fast procedure because of the substance in their cell walls.

Waxy

7

Non-lactose fermenters appear _______ on MacConkey agar.

Clear

8

____ media is one which facilitates distinguishing between types of microbes.

Differential

9

The color of the vegetative cell in the endospore stain.

Pink

10

A complex polysaccharide derived from seaweed which is added to nutrients to create a solid media.

Agar

11

What is the genus of bacteria that the Acid Fast stain designed to be used to detect?

Myobacteria

12

The color of the endospore after the endospore stain procedure shown in your notes.

green

13

If you used a basic dye such as crystal violet, and an acidic dye such as nigrosin on encapsulated bacteria, what would the appearance of the capsule be?

clear

14

What is the mordant used in the gram stain procedure?

Iodine

15

What is the decolorizing agent in the gram stain?

Alcohol

16

With the 10X ocular lens in place, what would be the total magnification if the 10X objective lens were used?

10X*10X= 100X

17

What is the counterstain used in the gram stain?

Safranin

18

Media wherein the exact amount of each chemical is known.

Chemically Defined

19

______ media is one which suppresses growth of some microbes while allowed growing of desired microbes.

Selective

20

Acid fast organisms appear what color after Acid Fast stain procedure?

Pink

21

Media which does not define exactly what nutrients are there.

complex media

22

Specialized, dormant structures that some types of bacteria can form under adverse conditions are known as _____.

Endospores

23

_____ is an example of a basic dye.

Crystal violet

24

____ and _____ are example of acidic dyes.

Nigrosin and India Ink

25

A _____enhances the stain's affinity for the cell.

Mordant

26

If you used a basic dye such as crystal violet, and an acidic dye such as nigrosin on encapsulated bacteria, what would the appearance of the bacterial cell itself be?

purple

27

If you used a basic dye such as crystal violet, and an acidic dye such as nigrosin on encapsulated bacteria, what would the appearance of the background around the cell be?

gray

28

MacConkey agar selects FOR gram _____ bacteria.

negative

29

What is the counterstain in the Acid Fast Stain?

Methylene Blue

30

Gram negative (-) cells appear what color after the gram staining procedure?

pink

31

Lactose fermenters appear _____ on MacConkey agar.

Pink

32

What is used as a mordant in the flagellar stain?

Carbolfuschin

33

The Acid Fast Stain is a ________stain.

Differential

34

____ dyes have a negatively charged colored ion.

acidic

35

Non acid fast organisms appear what color after the Acid Fast stain procedure?

blue

36

What is the primary stain in the Acid Fast Stain?

Carbolfuschin

37

This type of media is used to grow anaerobes because it is a reducing media which depletes the oxygen in the media.

Thioglycolate

38

The surface of a bacterial cell is normally ____ charged

negatively

39

_____ agar selects for gram negative bacteria and differentiates between lactose fermenters and non-lactose fermenters.

MacConkey

40

The _______ is a measure of the velocity of light as it passes through a specimen.

refactive index

41

What is the primary stain used in the gram stain?

Crystal Violet

42

The Gram Stain is a ______.

Differential

43

Gram positive (+) cells appear what color after the gram staining procedure?

Purple