Finals - Bio 14: Lab-3

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Microbiology
Chapters 11, 12, 22, 24, 26
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1

Consist of only species of microorganisms

Pure culture

2

More than one microorganisms growing in the culture

Mixed culture

3

Unwanted microorganisms

Contaminants

4

Use an inoculation loop and dip in the same sample then streak it across the petri dish in a zig-zag motion. The theory is that the bacteria will fall off one by one and grow into a colony. This is the most common isolation technique.

Streak plate technique

5

A small amount of diluted specimen is spread over a surface of a solid medium using a spread rod.

Spread plate technique

6

This is where you mix a small amount of diluted specimen into the melted agar and then pour it into an empty sterile petri dish. Organisms will grow on and throughout the agar.

Pour plate technique

7

Contain chemical that prevents the growth of unwanted bacteria without inhibiting the growth of desired bacteria.

Selective media

8

Contains a minimal amount of media to grow a particular organisms.

Minimal media

9

Contain chemicals that enhance the growth of the desired bacteria. Other bacteria will grow but the wanted bacterial growth will be enhanced.

Enrichment media

10

Contain various nutrients that allow one to distinguish one bacteria from another by how they metabolize the media with a waste product.

Differential Media

11

Bacteria that grows is gram negative

EMB

12

If metallic green colonies then it is __________

E. coli

13

If colonies are colorless then it is __________________

Ps. Aeruginosa

14

Contains high salt content that inhibits the growth of most organisms except for staphylococcus.

Mannitol agar

15

Bacteria that grows is gram positive

Mannitol agar

16

If turns yellow then it is _____________________

S. Aureus

17

If it turns medium white it is __________________

S. Epidermis

18

Is hot oven or incinerators. They denature enzymes, dehydrate microbes, and kills by oxidative effects. Standard application is 170 *C for 2 hours.

Dry heat

19

Is pasteurization, boiling and autoclaving.

Moist heat

20

Is 63-degree Celsius for 30 minutes or 72 degrees Celsius for 15 seconds killing pathogenic organism or those that cause spoilage?

Pasteurization

21

Is 100-degree Celsius for 10 minutes kills vegetative cells but does not inactivate endospores.

Boiling

22

The most effective method is ________________.

Moist heat sterilization or Autoclaving

23

Is psi at 121 C for 15 minutes and this usually kills endospores to render materials sterile.

Autoclaving

24

Chemicals used to control the growth of microbes.

Antimicrobial

25

Chemical agents used on innate objects to lower the level of microbes on their surface

Disinfectants

26

Chemicals used on living tissues to decrease the number of microbes

Antiseptics

27

results in bacterial death

bactericidal

28

cause temporary inhibition to bacterial growth

Bacteriostatic agents

29

against life (term used when something inhibits another)

Antibiosis

30

a substance produced by a microorganism that inhibits the growth of another.

Antibiotic

31

Disease-causing organism

Pathogens

32

Inhibits nucleic acid synthesis

Ciprofoxacin

33

Inhibits protein synthesis

Erythromycin

34

Inhibits cell wall synthesis by preventing cross-linking of peptidoglycan

Penicillin

35

Inhibits protein synthesis by interfering with attachment of tRNA carrying amino acids ribosomes.

Tetracycline

36

microorganisms that are more or less permanent.

Normal micro biota

37

microbes are present for only days or weeks

Transient Micro Biota