Finals - Bio 14: Lab-3
Consist of only species of microorganisms
More than one microorganisms growing in the culture
Use an inoculation loop and dip in the same sample then streak it across the petri dish in a zig-zag motion. The theory is that the bacteria will fall off one by one and grow into a colony. This is the most common isolation technique.
Streak plate technique
A small amount of diluted specimen is spread over a surface of a solid medium using a spread rod.
Spread plate technique
This is where you mix a small amount of diluted specimen into the melted agar and then pour it into an empty sterile petri dish. Organisms will grow on and throughout the agar.
Pour plate technique
Contain chemical that prevents the growth of unwanted bacteria without inhibiting the growth of desired bacteria.
Contains a minimal amount of media to grow a particular organisms.
Contain chemicals that enhance the growth of the desired bacteria. Other bacteria will grow but the wanted bacterial growth will be enhanced.
Contain various nutrients that allow one to distinguish one bacteria from another by how they metabolize the media with a waste product.
Bacteria that grows is gram negative
If metallic green colonies then it is __________
If colonies are colorless then it is __________________
Contains high salt content that inhibits the growth of most organisms except for staphylococcus.
Bacteria that grows is gram positive
If turns yellow then it is _____________________
If it turns medium white it is __________________
Is hot oven or incinerators. They denature enzymes, dehydrate microbes, and kills by oxidative effects. Standard application is 170 *C for 2 hours.
Is pasteurization, boiling and autoclaving.
Is 63-degree Celsius for 30 minutes or 72 degrees Celsius for 15 seconds killing pathogenic organism or those that cause spoilage?
Is 100-degree Celsius for 10 minutes kills vegetative cells but does not inactivate endospores.
The most effective method is ________________.
Moist heat sterilization or Autoclaving
Is psi at 121 C for 15 minutes and this usually kills endospores to render materials sterile.
Chemicals used to control the growth of microbes.
Chemical agents used on innate objects to lower the level of microbes on their surface
Chemicals used on living tissues to decrease the number of microbes
results in bacterial death
cause temporary inhibition to bacterial growth
against life (term used when something inhibits another)
a substance produced by a microorganism that inhibits the growth of another.
Inhibits nucleic acid synthesis
Inhibits protein synthesis
Inhibits cell wall synthesis by preventing cross-linking of peptidoglycan
Inhibits protein synthesis by interfering with attachment of tRNA carrying amino acids ribosomes.
microorganisms that are more or less permanent.
Normal micro biota
microbes are present for only days or weeks
Transient Micro Biota