5 Gene Expression: Transcription

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1

Messenger RNA is synthesized in the __ to __ direction.

A) 3` to 5`

B) 5` to 3`

C) All of the above

D) None of the above

B

2

Which statement(s) is/are true regarding transcription?

A) Messenger RNA is complementary and antiparallel to the DNA template strand.

B) DNA coding strand is identical to the mRNA.

C) DNA polymerases carry out transcription

D) None of the above

E) a and c are correct

A

3

How does transcription differ from replication?

A) RNA strand is attached to the DNA template in transcription, while the lagging DNA strand is not attached to the DNA template in replication.

B) RNAs are shorter than DNA.

C) Many copies of RNA are made from the same gene in a short time.

D) b and c are correct

E) All of the above are correct

D

4

RNA synthesis in eukaryotes is facilitated by which of the following? (select more than one answer)

A) RNA polymerase I

B) RNA polymerase IV

C) RNA polymerase II

D) RNA polymerase III

A, C, D

5

RNA polymerase I transcribes which gene type(s)?

A) rRNA genes

B) Protein-coding genes

C) snRNA genes

D) tRNA genes

E) All of the above

A

6

What are the differences between eukaryotic mRNAs and prokaryotic mRNAs? (select more than one answer)

A) Eukaryotic mRNAs must be modified for export out of the nucleus.

B) Eukaryotic genes contain introns that must be excised by splicing before export from the nucleus.

C) Prokaryotic mRNAs are monocistronic.

D) Eukaryotic mRNAs are polycistronic.

A, B

7

What is/are the purpose(s) of 5` capping in eukaryotic RNA processing?

A) Creates variation in protein code.

B) Allows mRNA to be exported.

C) Recognized by the small ribosome during translation initiation.

D) b and c are correct

E) All of the above are correct

D

8

What is/are the characteristic(s) of splicing?

A) Occurs in the nucleus and involves the excision of introns.

B) Splicing is carried out by the spliceosome.

C) Specific splice site sequences are present at intron/exon boundaries to direct splicing.

D) All of the above

E) None of the above

D

9

What is/are the difference(s) between RNA polymerase and DNA polymerase?

A) RNA polymerases do not have proofreading systems.

B) DNA polymerization can be initiated without a primer.

C) RNA polymerase reads one strand

D) a and c are correct

E) None of the above

D

10

Which cellular structure or organelle is responsible for assembling ribosomal subunits?

A) Nucleus

B) Mitochondria

C) Nucleolus

D) Lysosome

C