Final Test
7.2-7.8
normal pH of sperm
3.8-4.5
normal pH of vaginal secretions
greater than or equal to 2
Lecithin/sphingomyelin ration indicating fetal lung maturity
large gram positive rods
gram stain morphology of lactobacillus
between arachnoid and pia mater
location of CSF
clue cells
formed when numerous bacteria adhere to membranes of epithelial cells; most diagnostic indicator of bacterial vaginosis
occult blood
positive is a diagnostic indicator of colorectal cancer
lactoferrin
detects activated neutrophils in stool specimen
methylene blue
used to detect white blood cells in stool specimen
occult blood
used to detect red blood cells in stool specimen
2 hours
maximum amount of time a urine specimen can be left at room temperature before major changes begin to take place
pH and bacteria
chemicals that are increased in urine stasis
protein
chemicals that do not change in urine stasis
bilirubin, glucose, ketones, urobilinogen, blood cells, and casts
chemicals that are decreased in urine stasis
polarization
requires two filters: below condenser and analyzer between objective and eyepiece; splits into two beams when passing through; identifies birefringent substances such as crystals, fibers, bones, or minerals
CCPD calcium pyrophosphate
crystals present in pseudogout;
MSU monosodium urate
crystals present in gout
CCPD
perpendicular in polarizing microscopy
MSU
parallel in polarizing microscopy
first morning clean catch
preferred method of specimen collection
midstream clean catch
collection method used for bacterial cultures in order to reduce normal flora
1.3 million
amount of nephrons per kidney
nephron
the functional unit of the kidney
glomerulus
location of plasma filtration
600-1800mL
amount of urine excreted per day
collecting ducts
location of final concentration of urine decided
refractometer
tool used to measure specific gravity
1.002-1.034
normal specific gravity of urine
4.0-8.0
normal pH of urine
osmolality
affected by solute number not size or weight; determined in distal and collecting tubules when ADH is present; more accurate
proximal convoluted tubule
responsible for most reabsorption
loop of Henle
tubular portion of nephron immediately following and continuous with the proximal tubule located in the renal medulla
distal convoluted tubule
reabsorption and secretion of small molecules takes place; reabsorption of water to concentrate urine
collecting tubule
reabsorption of essential nutrients and water
Bowman's capsule
located within the glomerulus
anuria
absence of urine
oliguria
significant decrease less than 400mL per day
polyuria
a significant amount of urine greater than 3L per day
urea, chloride and sodium
three most prevalent solutes
urochrome
gives urine its characteristic yellow color
diabetes mellitus
condition leading to sweet or fruity odor of urine
uroeryhtrin
gives urine brick dust appearance
isothenuria
urine specific gravity is fixed at 1.010
1.000
specific gravity of distilled water
1.020
specific gravity of sodium chloride
nitroprusside
chemical reaction of ketone reagent pad
starving and vomiting and other lack of carb utilization
conditions in which ketones are found in urine
ictotest
detects bilirubin
SSA
detects protein
Acetest
detects ketones
Clinitest
detects sugars and reducing substances
ascorbic acid/vitamin C
high amounts can interfere and cause false positives
albumin
protein the reagent strip pad is most sensitive to
pseudoperoxidase
chemical reaction to detect blood
leukocyte esterase
azurophilic granules of leukocytes form and couple with diazonium salt
nitrite
forms diazonium salt which couples with compound in pad to produce azo dye; color change white to pink
glucose oxidase
double sequential; oxidizes glucose to form hydrogen peroxide and gluconic acid; peroxidase oxidizes chromogen in pad resulting in color change; specific for glucose
bilirubin
diazo reaction; diazonium salt in pad to form azo brown
hyaline
most common cast
distal and collecting tubules
where casts are formed
concentration, pH and flow of urine
factors that contribute to crystal formation
triple phosphate
coffin lid crystal
ghost cells
RBCs in hypotonic (dilute) urine
glitter cells
WBCs in hypotonic urine
degenerated blebs
WBCs in hypertonic urine
crentaed
RBCs in hypertonic (concentrated) urine
calcium carbonate
dumbbells
cholesterol
clear, thin parallelogram plates with notched corners
hypertonic
concentrated urine
hypotonic
dilute urine
amorphous urates
present in acidic urine; no clinical significance; dusting
acid urate crystals
salts; yellow-brown balls or spheres
uric acid
rhombic, diamond, or cube, barrel, or rosettes
monosodium urate
color-less to light-yellow slender, pencil-like prisms
calcium oxalate
most frequently observed crystals in human urine
calcium oxalate
ethylene glycol or ascorbic acid; envelopes
amorphous phosphate
alkaline urine; precipitate dusting
ammonium biurate
thorny apple appearance; seen after storage or in fresh renal tubular damage urine
calcium carbonate
tiny-colorless granular crystals in pairs or tetrads
bilirubin
small clusters of fine needles
cystine
colorless hexagonal plates uneven sides; acidic urine
upper UTI
casts commonly seen with increased leukocytes and bacteria
lower UTI
leukocytes and bacteria present in urine without casts
heavy proteinuria
associated with nephrotic syndrome
acute renal failure
sudden decrease in glomerular filtration rate; high mortality
chronic renal failure
progressive loss of renal function; hypertrophy of remaining healthy nephron
PKU
associated with musty urine
diabetes insipidus
polyuria associated with decreased ADH
galactosemia
screened for in newborns and tested with reducing substances tablet test
maple sugar urine disease
caramelized sugar odor of urine; mental impairment and spasticity in infants
diabetes mellitus type 1
insulin dependent diabetes; polyuria, ketonuria, and rapid weight loss
diabetes mellitus type 2
non insulin dependent diabetes; pancreas has decreased or insufficient insulin synthesis
porphyrias
hereditary defect of heme synthesis pathway; turns urine reddish; increased porphyrins and porphyrin precursors
MS
oligoclonal bands present in CSF but absent in serum
0.3-0.7
CSF to IgG index indicated acceptable blood brain barrier
CSF/Serum albumin index
used to assess permeability of blood brain barrier
monocytes and lymphocytes
white blood cells normal in small amounts in CSF
between arachnoid and pia mater
located of CSF
exudates
body fluids that are cloudy, various colors, can form clots
effusion
excess of fluid
centesis
performed to get rid of excess bodily fluid
chemistry, serology, and immunology
CSF#1
microbiology
CSF#2
cell counts and cytology
CSF#3
gram stain
look for bacteria and fungi
india ink
for Cryptococcus neoformans
acid-fast stain
for TB meningitis
Wright's stain
for ameba Naegleria fowleri
culture
to detect bacterial cause
detection of microbial antigens
to assist in identifying bacterial and fungal antigens in CSF