Microbiology: Exam 3 notes**

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Microbiology
Chapters 9-12
Microbiology 205 Fall 2012
updated 7 years ago by jenkarmata
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1

These are the 'hint' questions & information for Exam 3. Given by Mrs. Edelman

These are the 'hint' questions & information for Exam 3. Given by Mrs. Edelman

2

Fungi: are in the fungi kindom.

They are chemoheterotrophs & acuire food by absorption. With the exception of yeast, fungi are multicellular. Most reproduce w/sexual & asexual spores.

3

Fungi are

chemoheterotrophs

4

Fungi acquire nutrients by

absorption

5

With the exception of yeasts fungi are ____cellular.

multi

6

Most fungi reproduce with __________ spores

sexual & asexual

7

Algae: belong to SEVERAL kingdoms

* Can produce sexually & asexually
* they are photoautotrophs
* Produce several different photosynthetic pigments.
*Obtain nutrients by diffusion
* some are multicellular, forming colonies, filaments, or even tissues.
* A few produce toxins

8

Algae belongs to which kingdoms?

They belong to several

9

Algae are photoautotrophs or chemoheterotrophs?

PHOTOAUTOTROPHS

10

Algae produce several different _______ _______.

photosynthetic pigments

11

Algae obtain nutrients by

diffusion

12

Can algae produce toxins?

Yes. A few do produce toxins

13

Protozoa belong to several kingdoms

* most are chemoheterotrophic, but few are photoautotrophic
* obtain nutrients by absorption & ingestion.
*ALL ARE UNICELLULAR
* MANY ARE MOTILE
* Parasitic protozoans often form resistant cysts.

14

Protoza belong to _________ kingdoms.

several

15

Protozoa are

Chemoheterotrophic BUT few are photoautotrophic

16

Protozoa obtain nutrients by

absorption & ingestion

17

Protozoa are all ____cellular

UNI

18

Parasitic protozoans often form

resistant cysts

19

Many protazoans are (choose one) motile /non-motile

MOTILE

20

Q 1 pg. 261, 498, 718

Gel electrophoresis is

the separation of substances (such as serum proteins or DNA) by their rate of movement through an electrical field.

21

Q 3 pg 282, 284, 293, 303

Dichotomous Keys

An IDENTIFICATION scheme based on sucessive paired questions; answering one question leads to another pair of questions, until the organism is identified.
* WIDELY USED FOR IDENTIFICATION

22

Q4 pg. 293, 294 ex: pg 274, 280 or slide 10.19

How does a cladogram work?

A dichotomous phylogenetic tree that branches repeatedly, suggesting the classification of organisms based on the time sequence in which EVOLUNTIONARY branches arose.
* Each branch point, or node, in the cladogram represents an ANCESTOR COMMON TO ALL SPECIES BEYOND THAT NODE.

23

Q5
Staphoccoysus:

* Cells are in a cluster (like grapes)
* Is catalase POSITIVE
* Does not need enriched media for growth
* Are found on skin

24

Q5
Streptoccocus:

* Cells are in a string or chain
* Is Catalase NEGATIVE
* Needs enriched media for growth
* Are found in respitory tract.

25

Q7

What do they mean definitive vs. intermediate host?

Definitive- harbors sexually reproducing stages of Plasmodium.

Intermediate- The host in which the parasite undergoes asexual reproduction.

26

Q8

There are several types of algae that cause disease. Which one is not toxic?

Brown & green algae are not toxic.

27

Q9

What would you find with different...that are found in pool water?

*didn't get whole question.

Cryptospordium is a protozoan parasite in the phylum

28

Q10 pg. 346, 347

What is a dinoflagant?

They are UNICELLULAR algae collectively called plankton, or free-floating organisms. Their rigid structure is due to cellulose embedded in the plasma membrane. Some dinoflagellates produce neurotoxins.
* A TOXIC ALGAE

29

Dinoflagellates in the genus Alexandrium (in italics) produce

neurotoxins (called saxitoxins) that cause paralytic shellfish poisioning (PSP) & ciguatera.
* A TOXIC ALGAE

30

Q11 pg. 350, 351
What does an Entamoeba do?

Transmitted between humans through ingestion of cysts; excreted by feces. Found in human intestine.

31

Q12 pg. 350

Tropatazoma what does it do?

African sleeping sickness (T. brucei). T. Cruzi is trasmitted by the kissing bug (some cases in Tucson Az).

32

Q13 pg. 349-350

Giardia Lambia what does it do?

-has no mitochondria- & is sometimes called G. intestinalis or G. ductenalis. Excreted as a cyst in feces & survives in the environment before being ingested by next host. Diagnoses of giardiasis (disease caused by G. lambia) is often based on the identification of cyst in feces.

33

Q14

What amoeba goes to the brain?

Naeglria
* IF YOU DRINK A GLASS OF WATER INFESTED W/NAGELERIA, OR UNTREATED TAP WATER. A PERSON CAN GET INFECTED BY AMOEBA BY GETTING WATER IN THE NOSTRILS OR FECAL CONTAMINATION

34

Q15 pg. 353, 356

Balitinium coli- know about it

Is the only ciliate that is a human parasite. The causative agent of severe, though rare, type of dysentery. When the host ingests cysts, they enter the large intestine, into which the trophozoites are released.

35

Q16

What is Plasmodium?

A multiNucleated mass of protoplasm, as in plasmodial slime molds. * when written as genus, refers to the causative agent of MALARIA!!
Takes place in the liver & in the red blood cells of a human host.

36

Q16 pg. 247

Restriction enzymes:

as special class of DNA-cutting enzymes that exist in many bacteria.
The different cuts are:
Blunt ends- both strand are cut in the same place
Sticky ends- cuts that leave staggered ends

37

Cutting of Restrictive enzymes

recognizes & cuts, or digests, only one particulary sequence of nucleotide bases in DNA & it cuts this sequence IN THE SAME WAY EACH TIME.

38

DNA Polymers

Is a monomer of DNA nucleotides & polymer is knowN as polynucleotide.

39

Q17 pg. 253

DNA polymerase

Is a monomer of DNA nucleotides & polymer is known as polynucleotide.
*synthesizes a complementary strand of DNA, creating a double-stranded piece of DNA containing the information from the mRNA

40

pg. 251

Reverse Transcription

Is a genetic way of changing RNA to DNA
* PCR uses viral RNA or a cell's mRNA as the template. The enzyme, reverse transcriptase, makes DNA from RNA template, & the DNA is then amplified.

41

An artificial gene that contains only exons can be produced by using an enzyme called

REVERSE TRANSCRIPTASE to synthesize complementary DNA)(cDNA) from an mRNA template.

42

Pg. 248

Vectors:

Plasmids (most common vector) & viruses can be used as vectors. Most important property of a vector is the ability to self-replicate once in a cell.

43

Pg 249

Viral DNA:

A different kind of vector can usually accept much larger pieces of foreign DNA than plasmids can. After the DNA has been inserted into the viral vector, it can be cloned in the virus's host cells.

44

Pg 249

Suitable vectors:

depends on many factors, including the organism that will receive the new gene & the size of the DNA to be cloned. * must self-replicate once in a cell.

45

pg. 249

Plasmids

Plasmid are one of the primary vectors in use, particularly variants of R factor plasmids. Plasmid DNA can be cut w/the same restrictions enzymes as the DNA to be cloned, so that all pieces of the DNA will have the same sticky ends.

46

Polymerase Chain Reaction (PCR)

* to make multiple copies of a pieces of DNA enzymatically.
* to AMPLIFY DNA to detectable levels
* want to make multiple copies of DNA you need (PCR)
* cannot amplify an entire genome

47

Amplified DNA can be seen by..

gel electrophoresis.

48

In __a__ PCR, or __b__ PCR, the newly made DNA is tagged w/a fluorescent dye, so that the levels of fluorescence can be measured after every PCR cycle (that's real time aspect)

a. real-time

b. quantitative

49

RNA Polymers

Is an enzyme that produces RNA in cells.

50

What is DNA ligase?

Is an enzyme used to covalently link the backbones of the DNA pieces, producing an rDNA molecule.

51

Q18

Know about advantage for using ecoli as a human gene product

1. Human Insulin
2. Human growth hormone
3. Human blood clotting
4. Transgenic farm animals
* it is Gram-negative*

52

Q19 pg. 245 & 257

How is ecoli able to make insulin?

A gene from a vertebrate animal, including a human, can be inserted into the DNA of a bacterium, or a gene from a virus into a yeast may be used. In many cases, the recipient can then be made to express the gene, which may code for a commercially useful product.
* BY INSERTING THE INSULIN INTO A SUITABLE VECTOR, THE E.coli bacterial cell, TO PRODUCE AN INSULIN THAT IS CHEMICALLY IDENTICAL TO ITS NATURALLY PRODUCED COUNTERPART. This has been achieved using Recombinant DNA technology.

53

Pg. 257
Addition to 'how ecoli is able to make insulin.

Each of the 2 synthetic genes was inserted into a plasmid vector & linked to the end of a gene coding for the bacterial enzyme ß-galactosidase, & chemically joined to make human insulin

54

Q20 pg. 278

Correct way to write a scientific name of bacteria.

Capitalize the first letter & underline when writing or italicize when printing the whole name:
example: Salmonella truphimurium or S. truphimuirum (all in italics),

55

Q21

What features are only found in prokaryotes?

Lack of nuclear membrane- all prokaryotes are unicellular.

56

Q22

Know the purpose of arranging organisms into taxa

Putting organisms into categories to show degrees of similarties among organisms.
* Science of CLASSIFYING organisms
* Provides universal names
* Provides a reference for identifying organisms

57

pg. 286

Serology:

is the science that studies serum & immune responses that are evident in serum organisms are antigenic; that is, microorganisms that enter an animal's body stimulate it to form antibodies.

58

Q23 pg. 286-287

What is serological testing based on? What's the purpose?

-IDENTIFY BACTERIAL SPECIES & STRAINS BY determining their susceptiblitiy to various viruses (or phages)-
Can differentiate not only among microbial species, but also among strains within species. Serological testing was used to determine whether the increase in number of cases of necrotizing fascitis in the U.S & England since 1987 was due to common source of infections.

59

Q25

What is a clone?

A population of cells arising from a single parent cell.

60

Q26 pg. 290, 291f, 292

Nucleic acid hybridization- what is it?

If a double-stranded molecule of DNA is subjected to heat, the complementary strands will separate as the hydrogen bonds between the bases break. If the single strands are then cooled slowly, they will reunite to form a double-stranded molecule identical to the orgiinal double strand. (This renunion occurs because the single strands have complementary sequences.) WHEN THE ABOVE TECHNIQUE IS APPLIED TO SEPARATED DNA STRNDS FROM 2 DIFFERENT ORGANISMS, IT IS POSSIBLE TO DETERMINE THE EXTENT OF SIMILARITY BETWEEN THE BASE SEQUENCES of THE 2 ORGANISMS. THIS METHOD IS nucleic acid hybridiztion.

61

What is Nucleic acid hybridizations purpose

WHEN THE ABOVE TECHNIQUE IS APPLIED TO SEPARATED DNA STRANDS FROM 2 DIFFERENT ORGANISMS, IT IS POSSIBLE TO DETERMINE THE EXTENT OF SIMILARITY BETWEEN THE BASE SEQUENCES of THE 2 ORGANISMS. THIS METHOD IS nucleic acid hybridiztion.

62

Which bacteria’s produce oomycotes?

Oomycotes grow in fresh water that causes diseases to fish and terrestrial plants. It forms the cottony masses on dead algae and animals.
* so called water molds & downy mildews. They are filamentous protists which must absorb their food from the surrounding water & soil, or may invade the body of another organism to feed.
"oomycota" means "egg fungi" & refers to the large round oogonia, or structures containing female gametes

63

Pg. 249

Polymerase Chain Reaction (PCR)

a technique by which small samples of DNA can be quickly amplified, that is, increased to quantities that are large enough for analysis.

64

pg 300
Prokaryotic groups
Prokaryotes are grouped into 2 domains

1) Archaea

2) Bacteria

65

Alphaproteobacteria

includes most of the proterobacteria that are capable of growth at VERY LOW LEVELS OF NUTRIENTS.
* Some have unusual morphology, including protrusions such as stalks or buds known as prosthecae.

66

Alphaproteobacteria:

1. Pelagibacter
2. Azospirillum
3. Acetobacter & gluconbacter
4. *Rickettsia- Spotted fever / Rocky Mountain spotted fever.
5. *Ehrlichia- gram(-)transmitted by ticks, can be fatal.
6. Caulobacter & Hyphomicrobium
7. Rhizobium, Bradyhizobium & Agrobacterium
8. *Bartonella- gram(-) causes cat scratch disease
9. *Brucella- gram(-) invades our body's defenses WBC's
10. Nitrobacter & Nitrosomonas
11. Wolbachia

67

Ricettsia

gram (-) & rod shaped known as the spotted fever, transmitted by rat fleas.
*Obligate intra cellular parasites
*reproduce only within mammalian cells

68

Ehrlichia

gram (-)live obligately within WBC's. Transmitted by tick to humans & cause ehrlichiosis, SOMETIMES FATAL.

69

Bartonella

contains several members that are human pathogens. Best known is Bartonella henselae(italics),gram(-) bacillus that causes CAT SCRATCH DISEASE.

70

Brucella

Bacteria are small nonmotile coccobacilli. All species of Brucella (italics) are obligate parasites of mammals & cause the disease brucellosis. Brucella survive phahocytosis, an important element of the body's defense against bacteria.

71

Betaproteobacteria

often use nutrient substances that diffuse away from areas of anaerobic decomposition of organic matter, such as hydrogen gass, ammonia & methane.

72

Betaproterbacteria:

Thiobacillus
Spirillum
Sphaerotilus
*Burkholderia- Grows on hospital equipment & disinfectant solutions.
*Bordetella- whooping cough
*Pseudomonas
* Legionella
* Coxiella
* Vibrio
*= ones we need to remember for exam

73

Burkholderia

was formerly grouped w/the genus Pseudomonas, which isnow classified under gammaproteobacteria. Like the pseudomanads, almost all burkhlderia (italics) species are motile by single polar flagellum or tuft of flagella.
*grow on hospital equipment & disinfectant solutions.

74

Bordetella

gram (-) * whooping cough
Of special importance is the nonmotile, aerobic, gram (-)rod Bordetella pertussis(italics) This is serious pathogen is the cause of pertussis, or whooping cough.

75

Pseudomonas

*very important, consist of aerobic gram (-) rods that are motile by polar flagella cause,

76

Legionellales

common in streams & they colonize such habitats as warm-water supply lines in hospitals & water in the cooling tower of air conditions systems. Ability to survive & reproduce within aquatic amoebas often makes them difficult to eradicate in water systems.

77

Coxiella

Coxiella burnetii(italics) which causes Q fever
* requires a mammalian host to cell reproduce.
* not transmitted among humans by insect or tick bites
*TRANSMITTED BY aerosols or contaminated milk.

78

Vibrio

are rods that are often slightly curved. One important pathogen is Vibrio cholerae(italics) causative agent of cholera.
*profuse water diarrhea
*inhabits coastal salt waters it is transmitted to humans mostly by raw or undercooked shellfish.

79

pg 313

The Epsilonproteobacteria

slender gram (-) rods that are helical or curved. Motile by means of flagella & are microaerophilic.
*Campylobacter- one polar flagellum. C.fetus (italics) causes abortion.
*Helicobacter- curved rods w/multiple flagella most common for peptic ulcers & a cause of stomach cancer.

80

Mycoplasmatales

highly pleomorphic because they lack a cells wall & can produce filamints that resemble fungi, hence their name (mykes=fungus).
M. pneumonia, which is the cause of the common mild pneumonia.

81

Gardenerella

Garnerella vaginalis (italics) is a bacterium that causes one of the most common forms of vaginitis.

82

Actinomyces

consists of facultative anaerobes that are found in the mouth & throat of humans & animals. *actinomyces israelii causes actinomycosis, a tissue-destroying disease usually affecting the head, neck & lungs.

83

Nocardia

mophologically resembles actinomyces; however, these bacteria are aerobic. To reproduce, they form rudimentary filaments, which fragment into short rods.
can cause:
*chronic, difficult to treat pulmonary infection
*mycetoma- localized infection of feet or hands.
~common in soil.