Micro Bio Chp 7 Exam 3

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Microbiology
Chapter 7
Microbiology Kingwood Tx
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1

All of the following are associated with nucleic acid structure EXCEPT

ionic bonds

2

Which of the following is found at the 5" end of a DNA strand?

a phosphate group

3

The bacterial chromosome is

both circular and found in a nucleoid

4

Which of the following types of plasmids allows a bacterial cell to kill its competitors?

bacterioncin factors

5

Which of the following is NOT involved in the packaging of eukaryotic chromosomes?

Okazaki fragments

6

Which of the following statements is true of bacterial plasmids?

they are small circular DNA molecules that can replicate autonomously

7

The process indicated by the arrow in Fig 7.1 represents?

lagging strand synthesis

8

Which of the the following statements concerning transcription in bacteria is FALSE?

The same RNA polymerase transcribes primer RNA, mRNA, tRNA, and rRNA.

9

Which of the following is involved in translation?

mRNA, rRNA, and tRNA are all involved

10

Which of the following is a characteristic shared by DNA and RNA polymerase?

direction of polymerization

11

Typical eukaryotic genomes are composed of ______ DNA molecules.

multiple linear

12

A codon is a particular combination of three nucleotides. Therefore, there are _____ possible combinations of the nucleotides A, C, G, and T.

64

13

The AUG codon functions in coding for the amino acid methionine and as a

start signal

14

Which of the following regulatory RNAs alter translation in response to environmental conditions?

riboswitches

15

During elongation a charged tRNA first enters the ribosomal ______ site and then moves into the _____ site.

A, P

16

A wild-type organism and a genetic variant both have a gene encoding a receptor producing a chemotactic response to metabolite A. The genetic variant has a mutation in a regulatory gene for expression of the receptor. Which of the following statements with respect to the receptor is CORRECT?

The organisms have the same receptor genotype but different phenotypes.

17

Semiconservative DNA replication means that

each daughter DNA molecule is composed of one original strand and one new strand

18

Which of the following is NOT a characteristic of Okazaki fragments?

They are longer in eukaryotic cells

19

DNA replication and RNA transcription have many features in common. Which of the following does NOT occur in both processes?

a requirement for a primer

20

Which of the following processes is involved in the "central dogma" of genetic?

transcription and translation

21

Inducible operons

usually require an activator to be transcribed

22

Which of the following are considered to be frameshift mutations?

both deletions and insertions

23

If the codon AAA is changed to AAG, it still codes for the amino acid lysine; this is an example of a

silent mutation

24

Which of the following causes mutations by creating thymine dimers?

ultraviolet light

25

DNA damage caused by ethidium bromide results in _______ mutations.

both insertion and deletion

26

Which of the following is a DNA repair enzyme activated by visible light?

DNA photolyase

27

The Ames test demonstrates that a chemical is

mutagenic in Salmonella

28

The horizontal transfer process known as transduction

involves a virus

29

Frederick Griffith discovered

transformation

30

In conjugation, F+ cells

contain an F plasmid

31

Another term for the palindromic sequence found at the ends of transposons is a

inverted repeat

32

Which of the following is characteristic of prokaryotic genomes but NOT eukaryotic genomes?

circular chromosomes

33

The procedure known as "replica plating" is used in

negative selection only

34

The DNA sequence of a portion of gene in a wild-type organisms is TTACCATATTCACCC, and the corresponding peptide sequence is Leu-Pro-Tyr-Ser-Pro. The corresponding gene fragment in phenotypic variant of the organism is TTACCATATTCACCC and the peptide sequence is Leu-Pro-Cys-Ser-Pro. This is an example of a ______ mutation type resulting in a ______ mutation effect.

base substitution; missense

35

Which of the following is NOT involved in the regulation of the lac operon?

an iRNA

36

Genetic elements known as promoters are initiation points in the process of

transcription

37

Codons are recognized during

translation

38

The events of ______ are initiated at sequences called origins.

DNA replication

39

The process of ________ requires the activity of DNA ligase.

DNA replication

40

RNA polymerase is primarily responsible for

transcription

41

The process of ______ requires participation of tRNA molecules.

translation

42

The process known as _____ is initiated with the incorporation of fMet.

translation

43

Transfer of random pieces of DNA mediated by phage is known as

generalized transduction

44

The process of _______ is described as semiconservative.

DNA replication

45

During _____, a new peptide bond is formed with the amino acid located in the A site.

translation

46

A (operon/codon/gene) is a specific sequence of nucleotides that codes for a protein or an RNA molecule.

gene

47

Loosely packed, transcriptionally active regions of a eukaryotic chromosome is called (euchromatin/ heterochromatin/ nucleosomes).

euchromatin

48

Transfer of DNA between bacterial cells by viruses is called (transformation/ transduction/ conjugation).

transduction

49

The phenotype of an organism is its set of (genes/ traits/ chromosomes).

traits

50

The enzyme responsible for separating the DNA strands during DNA replication is (topoisomerase/ primase/ helicase).

helicase

51

The chemical 5-bromouracil mimics the chemical structure of thymine, making it an (analog/ nucleotide/ precursor) of thymine.

analog

52

A protein has altered function as a result of a single amino acid substitution in the polypeptide. this change resulted from a (missense/ nonsense/ silent) mutation.

missense

53

Except during intitiation of translation, transfer RNA molecules carrying amino acids initially bind to the ribosome at the (P/ A/ E) site.

A

54

The (codon/ antidcodon/ loop) of a transfer RNA molecule is complementary to a codon in a messenger RNA molecule.

antidcodon

55

Acridine dyes distort the structure of DNA causing (frameshift/ missense/ nonsense) mutations in gene sequences.

frameshiftf

56

A (genome/ codon/ operon) is a set of prokaryotic genes that are regulated and transcribed as a unit.

operon

57

The (leading/ lagging/ replicating) strand is the DNA strand that is synthesized continuously during DNA replication.

leading

58

The (phenotype/ genome/ genotype) is the set of genes in the genome of an organism.

genotype

59

RNA polymerase initiates transcription by recognizing specific DNA sequences called (promoter/ origins/ operons).

promoter

60

While studying a bacterial strain a scientist notes a short DNA sequences between inverted repeats in present in both the chromosome and a plasmid within the cell. This sequence is most likely a (phage/ transposon/ F plasmid).

transposon