Objectives: API20

Helpfulness: 0
Set Details Share
created 5 years ago by OceansVoice
100 views
updated 5 years ago by OceansVoice
show moreless
Page to share:
Embed this setcancel
COPY
code changes based on your size selection
Size:
X
Show:
1

Why is it necessary to perform a gram stain in conjunction with API20e testing?

must confirm that organism is a gram-negative rod

2

WHy is it necessary to perform an oxidase test in conjunction with API20e testing?

API20e is designed for organisms that do not have cytochrome c (oxidase negative)

3

Why are some cupules filled completely with DI water?

because these organisms require oxygen

4

Why is one tube sealed with mineral oil in the O-F test?

to allow for anaerobic growth and fermentation

5

Why would you extend the API20e test and use the 9 digit code?

if 7 digit code is not discriminatory enough after all reagents have been added

6

2 sets of procedures for continuing API20e test after initial incubation?

Oxidation-Fermentation Test
Motility Test

7

Why does the O-F medium include a high sugar-to-peptone ratio?

to reduce the possibility that alkaline products from peptone utilization will neutralize weak acids produced by the oxidation of the carbohydrate