Why is it necessary to perform a gram stain in conjunction with API20e testing?
must confirm that organism is a gram-negative rod
WHy is it necessary to perform an oxidase test in conjunction with API20e testing?
API20e is designed for organisms that do not have cytochrome c (oxidase negative)
Why are some cupules filled completely with DI water?
because these organisms require oxygen
Why is one tube sealed with mineral oil in the O-F test?
to allow for anaerobic growth and fermentation
Why would you extend the API20e test and use the 9 digit code?
if 7 digit code is not discriminatory enough after all reagents have been added
2 sets of procedures for continuing API20e test after initial incubation?
Why does the O-F medium include a high sugar-to-peptone ratio?
to reduce the possibility that alkaline products from peptone utilization will neutralize weak acids produced by the oxidation of the carbohydrate