Exercise 5-6 Micro Lab: Oxidase Test

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1

glucose is oxydized in ____

glycolysis

2

after oxydation what happens to glucose in glycolysis?

it is converted into two molecules of pyruvate and reduces two NAD to NADH

3

what happens to the pyruvate?

each pyruvate is oxidized and converted to a two carbon molecule called acetyl CoA and one molecule of CO2 which reduces another NAD and NADH

4

What happens in the KREBS cycle?

two more molecules of CO2 are produced per Acetyl CoA and reduces three more NADs and one FAD to FADH2.

5

What is the job of the electron transport chain?

to convert all the reduced coenzymes back to the oxidized state

6

2 functions of the electron transport chain

1. transport electrons down a chain of molecules with increasingly positive reduction potentials to the terminal electron acceptor

2. generate a proton motive force by pumping H+ out of the cell thus creating an ionic imbalance that will drive the production of ATP by way of membrane ATPases

7

_______, ________, and __________ are important ETC molecules unable to donate protons

Flavoproteins
iron-sulfur proteins
cytochromes

8

The last enzyme in the ETC, in Complex IV, is called _______

cytochrome C

9

chromogenic reducing agent

chemicals that develop color as they become oxidizes

10

what does cytochrome c oxidase have the ability to do?

to oxidize cytochrome c and catalyze the reduction of cytochrome c by a chromogenic reducing agent