Exercise 4-6 Micro Lab: Eosin Methylene Blue Agar

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1

EMB Agarq

a complex (chemically undefined), selective, and differential medium used for the isolation of fecal coliforms

2

EMB Agar contains what 5 things?

1. peptone
2. lactose
3. sucrose
4. eosin Y dye
5. methylene blue dye

3

In EMB, what does the peptone do?

provides a complex mixture of carbon, nitrogen, and other nutritional components

4

In EMB, why are sugars included?

they encourage growth of enteric bacteria to differentiate them based on color reactions created when combined with the dyes. Lactose supports coliforms such as E. coli and sucrose supports pathogens such as salmonella or proteus

5

What are the two purposes of the dye?

1. they inhibit the growth of gram positive organisms
2. they react with vigorous lactose fermenters and in the acidic environemnt, turn the growth deep purple or black