Exercise 4-2 Micro Lab: Columbia CNA with 5% Sheep Blood Agar

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1

Columbia CNA with 5% sheep blood

is a differential, undefined, selective media that allows growth of gram positive organisms and stops or inhibits growth of most gram negative organisms

2

Which 3 bacteria does the Columbia CNA help grow?

staphylococci, streptococci, enteroccocci

3

What 6 carbon energy sources are in the Columbia CNA?

1. casein
2. digest of animal tissue
3. beef extract
4. yeast extract
5. corn starch
6. sheep blood

4

In Columbia CNA, sheep blood provides the _________ and yeast extract provides the ________

X factor
B vitamins

5

What attacks gram negative bacteria in the Columbia CNA and how?

antibiotics colistin and nalidixic acid (CNA) interfere with DNA replication and affect membrane integrity

6

Which three bacteria are CNA antibiotics effective against?

1. Klebsiella
2. Proteus
3. Pseudomonas

7

How does sheep's blood help with the differentiation of gram positive organisms?

based on hemolytic reaction