Exercise 4-4 Micro. Lab: Mannitol Salts Agar

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1

Mannitol Salts Agar

A differential, selective media used for the differentiation and isolation of Staphylococcus aureus.

2

Mannitol Salts Agar is made up of what 3 things?

carbohydrate mannitol
7.5% sodium chloride
pH indicator phenol red

3

Phenol red is ____ at pH 6.8, ____ at 7.4 to 8.4 and ___ at above 8.4

yellow
red
pink

4

What does mannitol do?

provides the substrate for fermentation and makes the medium differential

5

What does sodium chloride do?

makes the medium selective because its concentration is high enough to dehydrate and kill most bacteria

6

Why do staph bacteria thrive in the mannitol salt agar?

because of their adaptation to salty habitats such as human skin