Exercise 5-12 Micro Lab: Starch Hydrolysis

Helpfulness: +1
Set Details Share
created 5 years ago by OceansVoice
244 views
updated 5 years ago by OceansVoice
show moreless
Page to share:
Embed this setcancel
COPY
code changes based on your size selection
Size:
X
Show:
1

Hydrolysis reactions

reactions that use water to split complex molecules

2

Hydrolytic enzymes

intracellular enzymes detected by a Urease Test that are required to catalyze hydrolysis reactions

3

intracellular

when the enzyme catalyzes its reaction inside the cell

4

extracellular (exoenzymes)

enzymes secreted from the organism to catalyze reactions outside the cell

5

gelatinase

an exoenzyme that liquefies the solid medium

6

Nutrient geletain detects what?

gelatinase

7

What 4 media are plated media that identify extracellular enzymes capable of diffusing into the medium and producing a distinguishable halo of clearing around the bacterial growth?

1. DNase Agar
2. Milk Agar
3. Tributyrin Agar
4. Starch Agar

8

starch

a polysaccharide made up of a-D-glucose subunits.

9

Starch exists in what two forms?

1. linear (amylose)
2. branched (amylopectin)

usually as a mixture with the branched configuration being predominant

10

Whats the deal with starch and the bacterial cell membrane?

Starch is too large to pass through it, so it has to split into smaller fragments or individual glucose molecules in order to be of any metabolic value

11

a-amylase
oligo-1, 6-glucosidase

these enzymes are able to hydrolyze starch by breaking the glycosidic linkages between sugar subunits.

12

starch agar

a simple plated medium of beef extract, soluble starch, and agar

13

What is used to detect the presence or absence of starch in the vicinity around the bacterial growth?

iodine

14

Iodine reacts with starch and produces what color?

a blue or dark brown color

15

starch agar was originally designed for cultivating _____

Neisseria

16

a clearing around the bacterial growth indicates what?

amalayse present