Exercise 2-13 Micro Lab: The Lethal Effect of Ultraviolet Radiation on Microbial Growth

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1

Ultraviolet radiation is a type of ___________

electromagnetic energy

2

UV travels in ____

waves

3

wavelength

the distance between adjacent wave crests and is typically measured in nanometers

4

Ultraviolet light is categorized into three groups:

1. UV-A
2. UV-B
3. UV-C

5

UV-A waves

the longest wavelengths, ranging from 315 to 400 nm

6

UV-B waves

wavelengths between 280-315 nm

7

UV-C waves

range from 100-280 nm

These wavelengths are most detrimental to bacteria

8

Why are UV-C waves the most detrimental to bacteria?

Bacteria exposure for more than a few minutes usually results in irreparable DNA damage and death of the organism

9

What is the most common use of UV light?

to disinfect laboratory work surfaces

10

gamma rays

The shortest and highest energy wavelengths

11

Gamma rays start at about ____nm

10^-5

12

How long are radio waves?

about a kilometer or longer

13

How long are ultraviolet light waves?

Between 100nm to 380nm

14

Two cultures used in lab today for comparing UV light

1. Bacillus subtilis
2. Escherichia coli