Acid fast staining lab 9
What is the purpose of heat during the acid fast staining procedure
Heat drives the carbolfuschin stain into the cells and once penetrated they are not easily decolorized by acid alcohol
What is the function of the counter stain in any differential stain procedure
Stains different bacteria that react differnt to diff dyes able to distinguish between bacteria
Are acid fast bacteria Gm pos or Gm neg? Explain
They are Gm positive because of lipid in the cell wall, they are stained by the primary stain
For what diseases would you use an acid fast stain
Tuberculosis, leprosy and other bacteria that have cell walls with lipids that stop dyes from readily binding to the cells
What makes an organism non acid fast
The absence of lipids (mycolic acids) iin cell walls
What chemical is responsible for the acid fast property of mycobacterium?
Mycolic acid, High lipid content
Is a gram stain an adequate substitute for an acid fast stain? Why or why not?
No b. these articular bacteria do not stain unless heated with carbolfuschin that drives pigment into cell