Lab exam 1

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Microbiology
Chapters 1-4
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1

Lab coat, gloves, open sink, autoclave

BSL-2

2

Lab coat, gloves, protective lab clothing, protective pressure personal suit, separate decontaminated facility.

BSL-4

3

primary lenses, magnify the specimen.

objective lense

4

magnifies the image formed by the objective lenses.

ocular lens

5

controls the amount of light.

diaphragm

6

light source

illuminator

7

transmits the image from objective lens to ocular lens

body tube.

8

spirogyra

algae

9

amoeba

protozoa

10

yeast

fungi

11

bacilli, cocci, spiral

bacteria

12

bio-safety level for most introductory microbiology labs

BSL-1

13

bio-safety level for clinical microbiology lab working with airborne pathogens.

BSL-3

14

metric unit used to express the size of most bacteria.

micrometer

15

lens used with immersion oil.

100x

16

why is immersion oil used?

prevent loss of resolution due to light refraction.

17

OOOOOO bacteria

Streptoccous

18

o0o bacteria

coco-bacilli

19

~~~~ bacteria

spirillum

20

hanging drop is used to study

motility

21

aseptic technique refers to?

carrying out a procedure with minimal chance of cross-contamination.

22

round colony shape

circular

23

spider colony shape

filamentous

24

advantage of growing bacteria on a slant

storage, easier to handle

25

branched growth pattern

arborescent

26

culture medium with know composition

chemically defined medium

27

culture medium with agar, peptone, and animal extract

complex medium

28

population of cells arising from a single bacterial cell.

colony

29

deep agar used for

motility and oxygen need of bacteria

30

bacterial growth layer at bottom of test tube.

sedimentation

31

sheet of bacterial growth

pellicle

32

cloudiness of broth

turbidity

33

inoculating needle

used for deep agar

34

inoculating loop

used to transfer bacteria to plate or slant.

35

incubation temperature

37*c

36

membrane filtration

used to sterilize culture mediums that are sensitive to heat.

37

incinerator/flame

used to sterilize inoculating loop/needel